论文标题:青海云杉花芽分化的生理生化研究
Physiological and Biochemistrical Study on Flower Bud Differentiation in Picea Crassifolia
论文作者
论文导师 汪晓峰；王军辉,论文学位 硕士,论文专业 生物化学与分子生物学
论文单位 北京林业大学,点击次数 147,论文页数 49页File Size4649K
2006-05-01论文网 http://www.lw23.com/lunwen_11184357/
Picea crassifolia; floral bud differentiation; ELISA; hormone; stem-injection; girdling; GA4/7
本论文采用了间接酶联免疫法,结合野外观察首次对我国青藏高原东北边缘特有树种——青海云杉生长发育过程中内源激素的动态变化进行了研究,为今后进一步研究其花芽分化的机理提供依据。同时,选取了9个无性系,进行了赤霉素(GA4+7)茎干注射,环割(girdling)和GA4+7+girdling处理。研究了不同处理对内源激素(GAs、IAA、ABA和ZR)含量变化的影响,为今后进行青海云杉促花促果奠定理论基础。 通过观察发现,青海云杉雌雄花芽每年4月底5月初开始芽开始萌动,到6月17号左右雄花开始散粉,大概一周左右,期间雌球果种鳞张开时间约3天,随即闭合;顶芽生长7月初即告封顶;封顶后到8月中旬为青海云杉花芽的生理分化时期;内源激素含量变化存在一定的相似性,全年中出现了两次高峰,分别出现再6月15号和8月3号左右,和花芽的形态分化和生理分化的起始时间刚好吻合;经过外源赤霉素处理的青海云杉植株以上两个峰值要明显高于对照,而当伴以环割处理时,这种增幅更加明显。同时发现和对照相比,环割处理导致植株内源ZR处在一个极低的水平。 高比率的ZR/GA, ZR/IAA, ZR/ABA是有利于青海云杉花芽生理分化的起始,但对维持花芽分化的进行可能不是必须的,而高比率的ABA/GA可能是花芽分化能够顺利完成所不可缺少的。
In this paper, the changes of endogenous hormones at the stage of flower bud differentiation in picea crassifolia was studied. In order to study more about the mechanism of flower bud differentiation; GA4+7 was applied alone or together with girdling by stem injection, effect on the change of endogenous hormones content was studied. It would be beneficial to the promotion of flower bud differentiation in picea crassifolia. It was observed that the time from May to the beginning of July was vegetative growth time, and then it would stop growing. From then to the middle of august was the physiological differentiation time. The results of study on changes of endogenesis hormone concentration show that GA, ABA and IAA both have two max value during the year growing time. ZR concentration was negatively correlated with flower initiation in many herbaceous species, also in Pinacea species. The results of study on the influence of stem injection with GA4/7 alone and in combination with girdling on changes of endogenesis hormone concentration show that both make the ZR concentration stay at a quite low level. While make the other 3 kinds of hormone’s max value much more bigger than control. The ratio of different hormone was also studied, the results show that high ZR/GA, ZR/IAA, ZR/ABA value was positively correlated with floral bud initiation. High value of ABA/GA is necessary for floral bud differentiation.
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