论文标题:天胡荽有效成分HSA抗肝纤维化作用及其机制研究
Protective Effect and Mechanism of HSA Against Liver Fibrosis
论文作者
论文导师 张如松,论文学位 硕士,论文专业 中药学
论文单位 浙江中医药大学,点击次数 286,论文页数 51页File Size9517K
2006-03-01论文网 http://www.lw23.com/lunwen_1156017/
HSA;chemical liver injury;anti-liver fibrosis; HBsAg; 2.2.15 Cell
Objective: (1) To isolate the active compound from the extraction of Hydrozotyle sibthorpioides Lam. (2) To prove the inhibitive effect of HSA against carbon tetrachloride (CCl_4)-induced liver fibrosis in rats. (3) To study protective effect and involved possible mechanisms of HSA. Methods: (1) Isolation of active compound: Subject the fraction to column chromatography on silica gel, and two compounds were obtained.Seive the active cmpound by chronic liver injury experiment. (2) The models of CCU-induced liver fibrosis in rats wer used in the experiment.The experimental rats were divided into 6groups: untreated, CCU control, Tetrandrine group, and three HSA groups(containing HSA 1.5g/kg, 0.5g/kg, 0.1g/kg weight respectively). Alanine transaminase(ALT) 、 aspartate aminotransferase(AST)、albumin(Alb) and total protein(TP) in serum and Hydroxyproline(Hyp) in liver were measured. We also observed the changes of liver histopathology to prove the antifibrosis effect of different doses of HSA. (3) Anti-virus experiment invitor: Using the HBV genetic transfection hepatic cell of human-2.2.15 cell as cellular model to screen anti-HBV drugs. To observe the effect of HSA on the 2.2.15 cell secreting HBeAg& HBsAg using SPRIA and test its toxicity on hepatic cell with MTT chrornometry. Anti-acute liver injury: The models of TAA-induced acute liver injury in mice and 5 doses of HSA(2g/kg, 1g/kg, 0.5g/kg, 0.25g/kg, 0.125g/kg)were used to study the protective effect and involved possible mechanisms of HSA on liver injury. Results: (1) We obtained two
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