论文标题:大黄质量控制及复方大青叶注射液指纹图谱方法研究
Study on the Method of Quality Control for Radix et Rhizoma Rhei and the Fingerprint Analysis of Injectio Folii Isatidis Composita
论文作者
论文导师 李发美,论文学位 硕士,论文专业 药物分析学
论文单位 沈阳药科大学,点击次数 306,论文页数 99页File Size2420K
2004-05-01论文网 http://www.lw23.com/lunwen_1212867/
Radix et Rhizoma Rhei; Injectio Folii Isatidis Composita; HPLC; Chromatographic fingerprint; Chemical pattern recognition
复方大青叶注射液由大青叶、金银花、大黄、羌活、拳参5味药材加工制成，本论文以其中的大黄药材为研究对象，对其质量控制方法进行研究，并初步建立了复方大青叶注射液的高效液相色谱指纹图谱分析方法。 采用高效液相色谱法同时测定19批不同种及来源的大黄药材中五个活性成分芦荟大黄素(aloe-emodin)、大黄酸(rhein)、大黄素(emodin)、大黄酚(chrysophanol)、大黄素甲醚(physcion)的含量，其色谱条件为：Kromasil ODS-1色谱柱，流动相为甲醇-水-冰醋酸(90:10:0.4，V:V:V)，流速为0.8mL·min~(-1)，检测波长254nm，所有成分在20min内可达到完全分离，每种成分在各自的浓度范围内均具有良好的线性。对19批不同种及来源的大黄药材醇提液进行了HPLC指纹图谱分析，获得反映药材化学成分信息的数据，对其进行系统聚类分析，将样品分为6类，以Ⅱ类药材所得的色谱数据为模板，采用夹角余弦作为测度，进行相似度计算。结果表明优质品相似度在0.90～1.00之间，一般品相似度在0.70～0.90之间，非正品相似度小于0.61。同时进行了各种大黄药材的抑菌活性试验，用相关分析揭示色谱数据和抑菌圈直径之间的关系，得出与抑菌活性呈正相关的7个化学成分。 采用反相高效液相色谱法对复方大青叶注射液进行指纹图谱分析，共获得57个色谱峰，结合主成分分析投影判别法比较了同一企业不同批次和不同企业同一产品的化学指纹差异。采用反相高效液相色谱法测定复方大青叶注射液中大黄素和大黄素甲醚的含量，其色谱条件为：Kromasil C_8色
Injectio Folii Isatidis Composita is prepared from Folium Isatidis, Flos Lonicerae, Radix et Rhizoma Rhei, Rhizoma et radix notopterygii and Rhizoma Bistortae. In this thesis, quality control method of Radix et Rhizoma Rhei was developed. High performance liquid chromatography(HPLC) was employed in fingerprint analysis of Injectio Folii Isatidis Composita.Five bioactive constituents namely aloe-emodin, rhein, emodin, chrysophanol, physcion in 19 samples were determined with high performance liquid chromatography. The samples were separated on an ODS column with the mobile phase of methanol-water-acetic acid (90:10:0.4, V:V:V). The flow rate was 0.8 mL·min~(-1), and the detection was set at 254 nm. All constituents were completely separated in 20 min. The chromatographic peak areas presented a good linearity with the concentration for all constituents. The chemical variables of Radix et Rhizoma Rhei as a whole were obtained by HPLC fingerprint analysis and 40 peaks were detected. The 19 samples were divided into 6
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