论文标题:外伤性视网膜脱离中M(?)ller细胞的免疫组织化学观察
Immunohistochemical Observation of M(?)ller Cell following Traumatic Retinal Detachment
论文作者 马汀
论文导师 马志中,论文学位 硕士,论文专业 眼科
论文单位 中国人民解放军军医进修学院,点击次数 238,论文页数 52页File Size1953k
2002-06-01论文网 http://www.lw23.com/lunwen_1267822/ 视网膜脱离;外伤;动物模型;Muller;细胞;GFAP;GS;免疫组织化学
retinal detachment;trauma;model;Mtlller cell;GFAP;GS;immunohistochemistry
目的： 1．建立非外源性诱导物引发的外伤性视网膜脱离动物模型。 2．通过对兔外伤性视网膜脱离后不同时间GFAP、GS等特征性抗原表达的动态观察，探讨Müller细胞在外伤性视网膜脱离中的功能变化和作用。 方法： 1．兔眼后上方角膜缘后2mm平行切开巩膜，挤压出玻璃体，并向神经上皮层下注入眼用BSS，造成视网膜180°脱离，8—0可吸收线缝合伤口。以此制造外伤性视网膜脱离的动物模型。 2．组织病理学光镜及电镜观察外伤性视网膜脱离后视网膜的组织学改变。 3．利用免疫组织化学方法检测视网膜脱离后不同时间视网膜中GFAP、GS的表达。 结果： 1．视网膜脱离后视网膜各层出现不同程度的退行性改变，以光感受器受损最为显著，神经元细胞成分减少、结构破坏，Müller细胞出现增生、肥大及胶质化等改变。 2．正常视网膜及伤后1天，GFAP表达呈阴性；伤后3天，GFAP阳性表达开始出现，随视网膜脱离时间的延长，GFAP的阳性表达逐渐增强。 军医进修学院 硕士学位论文 中文摘要 3刀S在正常视网膜中呈强阳性表达，随视网膜脱离时间的 延长，其阳性表达呈现递减趋势。 结论： 1．此模型视网膜脱离发生率高，后期有PVR的发生，可用。于视网膜脱离引发PVR的研究。 2．视网膜脱离发生后，Milller细胞经历了增生、肥大、纤 维支架结构改变等组织学改变，对视网膜的支持、营养作用和 对神经元的调制功能出现不同程度减退，提示Milller细胞的胶 质化增生可能在视网膜脱离后的异常修复过程中起重要作用。
Purpose: 1.To establish the model of traumatic retinal detachment in the rabbit and to evaluate the function and role of Muller cell in experimental retinal detachment by the method of pathology and immunohistochemistry.Method: 1. BSS injection into subretinal space via a silicon tube after scleral incision and vitreous removal was made for the model of traumatic retinal detachment in rabbits. 2. Morphological observation was carried out with light microscope and electron microscope after experimental retinal detachment at different time intervals. 3.Detection of immunoreactivity of Glial Fibrillary Acid Protein (GFAP) and Glutamine Synthetase(GS) following experimental retinal detachment using the method of immunohistochemistry.Result: 1. Under light microscope and electron microscope, an entensive loss of photoreceptors accompanied by neural retina degeneration were found. Proliferation, hypertrophy and gliosis of Muller cells could be observed. 2. GFAP was undetectable in Muller cells at 1 day after detachment. There were an aggregation of GFAP in the endfoot of Muller cells at 3 days .At 28 days after detachment, GFAP could be detected within the subretinal space, formed glial scars. 3. GS was expressed in both normal and detached retinas at different time intervals, and immuno-staining decreased with prolonged detachment.Conclusions: 1. Traumatic retinal detachment was successfully induced in the rabbit. PVR was well developed in the long-term tinal detachment. 2. After retinal detachment GFAP expression increased while GS decreased in terms of intensity and distribution. 3. Malfunction of Mtlller cells in sustainment, nutrition and modulation of neurons of retina, in the form of gliosis, may play a significant role in abnormal recovery process of detached retina.
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