论文标题:高血压大鼠脑缺血再灌注的病理及药物对HSP_70干预的研究
THE STUDY OF PATHOLOGY AND HSP_70 EXPRESSION INTERFERED BY DRUGS OF HYPERTENSIVE RATS AFTER MCAO AND REPERFUSION
论文作者 石胜良
论文导师 毕桂南;莫雪安,论文学位 硕士,论文专业 神经病学
论文单位 广西医科大学,点击次数 58,论文页数 45页File Size1311k
2001-05-01论文网 http://www.lw23.com/lunwen_127719277/ 高血压;大鼠;热休克蛋白70;脑保护
hypertension;rat;HSP70;cerebral protection
目的 探讨急性脑缺血再灌注损伤的病理改变，某些药物对HSP_(70)表达的影响及其脑保护作用。 方法 将大鼠随机分为两组：一、高血压组：将大鼠行双肾动脉狭窄术制成肾性高血压大鼠模型。术后喂养2个月，并分批测量其血压。二、对照组：本组大鼠不做双侧肾动脉狭窄术，与高血压组大鼠喂养相同时间。然后再将高血压组大鼠随机分为三组：1、测体积组，又分为：西比灵组、妥泰组、缺血组、假手术组。2、病理及HSP_(70)组：同样也分为西比灵组、妥泰组、缺血组、假手术组。3、HSP_(70)表达观察组：缺血后再灌注时点分别为0h、3h、6h、12h、1d、3d、7d。以上3组MCAO时间均为2h。再灌注时点：测体积组为6h；病理及HSP_(70)组为1d。测体积组将鼠脑经TTC染色后，用计算机病理图象分析仪测量出梗死面积，根据梯形法则计算出梗死体积及梗死体积比。测量HSP_(70)组，经免疫组化染色后用病理图象分析仪测出HSP_(70)阳性细胞数。 结果 1、行双肾动脉狭窄术的大鼠经检查，血压升高，且有4例出现自发性卒中；2、制备MCAO及再灌注模型的大鼠均出现对侧偏瘫症状；3、用药组HSP_(70)的表达高于单纯缺血组；4、用药组脑梗死体积比明显小于单纯缺血组。 结论1、双肾动脉狭窄法制备易卒中高血压鼠模型是一种行之有效的方法；2、改良Longa、法制备MCAO再灌注模型切实可行；3、西比灵和妥泰能促进HSP70的表达，使梗死体积减小，对缺血再灌注引起的受损脑组织起保护作用。
THE STUDY OF PATHOLOGY AND HSP70 EXPRESSION INTERFERED BY DRUGS OF HYPERTENSIVE RATS AFTER MCAO AND REPERFUSIONABSTRACTObjectives To study the pathology of acute cerebral ischemic reperfusion damage and the affection of some drug to the expression of HSP70 and its cerebral protection afterischemic reperfusion. Methods Rats were distributed to two groups: 1.Hypertension group: Double renal artery stenosis of the rats were performed. After this operation, the rats of this group were fed for two months. Then measured their blood pressure. The rats whose blood pressure were high were separated into three groups. (1) group measured volume: consisted of sibelium group, topamax group, single ischemic group, fake operation group and normal rat group. (2) Pathology and HSP?o group: sibelium group, topamax group, single ischemic group, fake operation group. (3)HSP7o dynamic observation group: The reperfusion time point after MCAO were:0h,3h,6h,12h,ld,3d,7d. The MCAO time of all three groups above was two hours, but the reperfusion time was different: the first group was six hours; the second was one day. The rat brains of the group measured volume were dyed with TTC and measured the area by computerized pathological image analyzer. Then the infarct volume and the ratio of infarctvolume were calculated. In HSP70 group, the samples were stained using immunohistochemistric method. The number of positive cells and the stained intensity were measured by computerized pathological image analyzer. 2 control group: the rats of this group were not performed the operation of double renal artery stenosis. The feed time was equal with group one.Results l.The blood pressure of the rats which were operated double renal artery stenosis was high, and four rats suffered stroke. 2.The rats which were performed the operations of MCAO and reperfusion all had right limb parelysis.S.The HSP70 expression of groups using drugs were higher than single ischemic group. 4.The infarct volume of groups using sibelium and topamax were smaller than that of single ischemic group.Conclusions l.The method of double renal stenosis is effective to make RHRSP.2.The improved Longa"s method making MCAO and reperfusion model is practicable. 3.Sibelium and topamax can enhance the expression of HSP70; decrease the infarct volume and protect the ischemic cerebral tissue after MCAO and reperfusion.
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