论文标题:肌球蛋白轻链激酶表达水平与炎症性肠病活动关系的研究
The Research about the Relationship between Myosin Light Chain Kinase Expression and Inflammatory Bowel Disease Activity
论文作者
论文导师 高敏,论文学位 硕士,论文专业 内科学
论文单位 浙江大学,点击次数 80,论文页数 35页File Size2178K
2007-04-01论文网 http://www.lw23.com/lunwen_170798312/
myosin light chain kinase;inflammatory bowel disease; epithelial tissue; immunohistochemical staining
背景：在炎症性肠病中，患者的肠上皮组织屏障往往遭受破坏，而这可能会促进炎症性肠病的发生和进展。体外实验研究表明肌球蛋白轻链激酶表达增加能介导上皮组织屏障功能障碍，而该功能障碍是由细胞因子诱导的。 肠上皮的一个基本功能就是作为一个屏障，防止不可调节的混合物进入到细胞之间的间隙和间质中。与此同时，有关上皮组织屏障功能障碍研究是目前开展的比较活跃，普遍认为，缺乏抵抗力的屏障是感染性、缺血性和免疫介导肠疾病的共同特征。 研究目的：探讨肌球蛋白轻链激酶(MLCK)在炎症性肠病中的表达。 方法：选择本院2006年-2007年2年来病理诊断为克罗恩病10例和溃疡性结肠炎10例，对他们的肠镜活检病变组织石蜡块进行切片；选取10位行结肠镜检查病人的正常结肠组织各一块，进行组织切片。克罗恩病和溃疡性结肠炎的组织切片为试验组，正常肠组织切片为对照组，分别进行免疫组化试验以检测上皮组织MLCK表达量，用图象分析系统采集显微镜下图象，计算阳性率。 结果：克罗恩病组的阳性率为50.0％±10.0％，溃疡性结肠炎组的阳性率为57.6％±25.7％，正常对照组的阳性率为9.2％±2.7％。克罗恩病组的阳性率明显高于正常对照组，其差异有显著统计学意义(P＜0.01)；溃疡性结肠炎组的阳性率明显高于正常对照组，其差异有显著统计学意义(P＜0.01)。 结论：炎症性肠病病变上皮组织的MLCK表达高于正常肠组织。
Background:The intestinal epithelial barrier is frequently disrupted in inflammatory bowel disease (IBD) and this has been proposed to play a role in disease pathogenesis and reactivation. In vitro studies show that cytokine-induced epithelial barrier dysfunction can be mediated by increased myosin light chain kinase (MLCK) expression.A primary function of intestinal epithelia is the maintenance of a barrier to prevent unregulated mixing of luminal and interstitial compartments. While the relevance of epithelial barrier dysfunction to disease pathogenesis remains a subject of active investigation, it is well accepted that compromised barrier function is a common feature of infectious, ischemic, and immune-mediated intestinal disease.Objective: To discuss myosin light chain expression in inflammatory bowel disease.Methods: Biopsy specimens were obtained from patients undergoing colonoscopy for IBD,10from ulcerative colitis and 10 from Crohn"s disease. Control samples were obtained from patients undergoing colonoscopy as part of a routine program of polyp surveillance. The number of control samples is 10.Then to detect the myosin light chain expression,the above samples are in immunohistochemical staining.At last we calculate the rate of positive cells through the microscope.Results: The rate of positive cells in Crohn"s disease is 50.0% ±10. 0%, the rate of positivecells in ulcerative colitis is 57.6%±25. 7%, the rate of positive cells in control samples is 9. 2%±2.7%. the difference between Crohn"s disease and control samples is significant., (P<0. 01) sois the difference beween ulcerative colitis and ulcerative colitis. (P<0. 01)Conclusion: epithelial myosin light chain kinase expression is upregulated in inflammatory boweldisease.
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