论文标题:两个太湖粳稻地方品种抗稻瘟病基因分析
The Analysis of Blast Resistance Genes in two Taihu Lake Japanica Rice Varieties
论文作者 何新建
论文导师 张红生,论文学位 硕士,论文专业 作物遗传育种
论文单位 南京农业大学,点击次数 73,论文页数 50页File Size1641k
2000-06-01论文网 http://www.lw23.com/lunwen_194203457/ 太湖粳稻；稻瘟病；抗性遗传；抗病基因；等位性测定
Taihu japanica rice; blast;resistance genetic;disease resistance gene; allelic test
采用苗期喷雾接种和成株期注射接种法，比较了两个太湖地区粳稻地方品种黑壳紫粳和薄稻，一个云南粳稻地方品种丽江，以及12个日本抗稻瘟病单基因鉴别品种等15个品种，对7个日本稻瘟病菌标准鉴别菌系和我国两个太湖地区优势小种ZG_1、ZE_3的抗性反应。结果表明，黑壳紫粳对供试的9个菌系都表现抗病；薄稻除对研53-33表现感病外，对其它8个菌系表现抗病。日本抗稻瘟病单基因鉴别品种藤坂5号（Pi-i）、福锦（Pi-z）、砦1号（Pi-z~t）、K1（Pi-ta）和K59（Pi-t）对太湖地区优势小种ZG_1和ZE_3均表现抗病性。 黑壳紫粳与丽江杂交的F_1、F_2世代以及由F_2单粒传产生的F7家系分别接种日本鉴别菌系研54-04和北1，根据抗感分离比例和X~2测验，初步明确黑壳紫粳对菌系研54-04的抗性是由两个显性抗病基因控制的，对菌系北1的抗性是由一个显性抗病基因控制的。抗病基因的抗病谱分析和等位性测定的结果表明：控制对菌系研54-04抗性的一个抗病基因可能为已知抗病基因Pi-i；而另一个抗病基因同时控制了对菌系北1的抗性，它与日本抗稻瘟病单基因鉴别品种中对北1具有抗性的基因是不等位，可能为新基因。 薄稻与丽江杂交的F_1、F_2和回交世代分别接种菌系研54-04、ZG_1，发现薄稻对研54-04的抗性是由两个显性抗病基因控制的；对ZG_1的抗性是由一个显性抗病基因控制的。
The reaction of 12 Japanese different varieties with a single known blast resistance gene and two resistance Taihu japanica rice landraces, Baodao and Heikezijing, to the strains used in this experiment is researched. The analysis of the inheritance of these two vaneties resistance to the Japanese representative strains and the local dominant M grisea races, Z01 and ZE3, was carried out. Results indicate that Baodao is resistant to the eight used strains except for ken53- 33 and Heikeing is resistant to all used strains. Among the known blast resistance genes in the Japanese different varieties, P1-i, Pi- N-z, P1-ta awl Pi-t show resistant to both of ZG1 and ZE3. These guns may be applied in the local blast resistance breeding program. The F1, F2 and B,F, from the cross of Baodan and Lijiang were obtained. The materials of these generations were inoculated with two races, Kcn54-04 mad ZG1, which indicated that the resistance variety, Baodao, has two germ that condition the resistance to the two strains respectively. The genes that condition the two different strains is not the same and perhaps have tighten linkage relatiomhips. F1, F2 awl RILs from the cross of Heikuijing mid Lijmng wu inoculated by the two strains, KenS4-04 and Hokul. Results showed that the resistance of Heikezij lug to Ken54-04 and Hokul is respectively conditioned by two genes awl one gene. One of the two resistance genes that comlition the resistance to KenS4- 04 is the gene which conditions the resistmie to Hokul. The F2 from the cross of Heikezijing and the Japanese different varieties were inoculated with Hokul. results indicated that the resistance gene to Hokul is mmalielic with known resistance genes, and it is a new gene compared with the known guns in the Japanese different varieties, which was tentatively named after Pi-hei. The other blast resistance gene in Heikezij lug can be deduced to be the known gene, P1-i.
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