论文标题:游泳运动对大鼠心血管系统肾上腺髓质素mRNA及其受体活性修饰蛋白mRNA表达的影响
The Effect of Swimming Exercises on the Expression of Cardiovascular ADMmRNA and RAMP2mRNA in Rats
论文作者 贺杰
论文导师 许豪文,论文学位 硕士,论文专业 运动人体科学
论文单位 华东师范大学,点击次数 111,论文页数 47页File Size1912k
2004-05-01论文网 http://www.lw23.com/lunwen_2071302/ 游泳运动,ADM,RAMP2,CGRP
swimming exercises,ADM,RAMP2,CGRP
从分子结构的同源性和生理功能的相似性上看，肾上腺髓质素(ADM)和降钙素基因相关肽(CGRP)都是降钙素基因相关肽超家族的重要成员，都能很强地扩张血管，降低血压，并在增加心肌收缩力上可能起一定的作用。ADM和CGRP舒张血管的机制在于通过内皮细胞或血管平滑肌细胞表面的膜受体实现跨膜信号传递，比较奇特的是，该受体可以在不同修饰蛋白(RAMPs)的作用下识别降钙素基因相关肽超家族中不同的信号分子。比如RAMP2对降钙素受体样受体(CRLR)的修饰能使之成为ADM的受体。有关ADM及其受体的研究国内外仅限于医学、生物学方面的报道，至于ADM及其受体在急性运动、慢性运动训练过程中基因表达的变化，尚未见报道。长期的运动训练能给心血管系统带来不同结构层次上的良好适应，本研究主要探讨运动训练带来心血管系统ADM、CGRP、RAMP2在mRNA水平上的变化，并且就这种变化对运动适应的分子机理作出初步的解释。 实验选用健康纯系雄性Spra邵e一nawley(SD)大鼠24只，体重加0一250克，随机分为三组:安静对照组(CR，n二8)、运动力竭组(ER，n=8)、运动训练组(TR，n=8)进行饲养训练。之后分别取心尖肌组织和主动脉弓部血管测定ADMmRNA、CGRP mRNA、RA加IpZmRNA的表达水平。结果如下: (1)和安静对照组相比，一次急性有氧力竭游泳运动对大鼠心脏和主动脉ADM、RAMPZ和CGRP的mRNA表达无显著影响。 (2)和安静对照组相比，长时期进行低强度的有氧游泳训练能在不同程度上引起心脏ADM、RAMPZ和CGRP的mRNA表达显著上调，反映出长期低强度的有氧运动训练后心脏在分子水平上的良好适应;而长时期进行低强度的有氧游泳训练对主动脉的影响是ADMmRNA表达显著下调，RAMPZ和CGRP的mRNA表达无显著变化。
Based on the structural homogeneity and similarity in physiological effect, adrenomedullin(ADM) and calcitonin gene-related peptide (CGRP) are the important members of the CGRP superfamily, both of which can potently dilate vessel, decrease blood pressure and perhaps play a partial role in the strengthened contraction of cardiac muscle. The mechanism by which ADM and CGRP dilate vessel and potentiate contraction of cardiac muscle is that trans-membrane signal transduction is facilitated by receptors distributing in the membrane of vessel smooth muscle and cardiocyte. To our surprise, modified by different transmembrane activity modifying proteins(RAMPs), the same receptor binds different signal molecules of the CGRP superfamily. For example, calcitonin receptor-like receptors(CRLR) can be modified by RAMP2 to became ADM particular receptor. Published research reports on ADM and receptors areconfined in the fields of medicine and biology. Heretofore, little is known about the effect of acute exercise and long-term training on the expression of ADM and receptors. Long-term sports training induces benign cardiovascular adaptation in every structural level. The main purpose of the research is to survey rats cardiovascular expression of ADMmRNA CGRPmRNA RAMP2mRNA after a eight-week swimming training, and then to elucidate elementarily molecular mechanism of exercise-induced adaptation.24 purebred male Sprague-Dawley rats are divided randomly into three groups: Controlled (CR, n=8) , Exhausted (ER, n=8) ,Trained (TR, n=8) . After swimming training left ventricular muscle and main artery arch are extracted, we are inspecting the expression of ADMmRNA CGRPmRNA RAMP2mRNA by RT-PCR. The results are as follows:1. Comparing with the controlled group,once exhausting exercise has no significant effect on cardiovascular expression of ADMmRNA, RAMP2mRNA and CGRPmRNA.2. Comparing with the controlled group,long-term aerobic swimming training upregulates significantly cardiac expression of ADMmRNA,RAMP2mRNA and CGRPmRNA, suggesting that long-term aerobic training induces benign cardiac adaptation in molecular level; nevertheless, long-term aerobic swimming training downregulates significantly vascular expression of ADMmRNA, and has no significant effect on vascular expression of RAMP2mRNA and CGRPmRNA.
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