论文标题:粳稻沈农606抗稻瘟病基因分子标记的建立
Development of SSR Molecular Markers Linked to the Rice Blast Resistance Gene in SN606
论文作者
论文导师 钟鸣,论文学位 硕士,论文专业 生物化学及分子生物学
论文单位 沈阳农业大学,点击次数 82,论文页数 73页File Size7636K
2006-05-10论文网 http://www.lw23.com/lunwen_210805552/
Rice; Rice blast disease; SSR marker; Resistance gene; Gene mapping; Molecular marker-assisted selection
由子囊菌Magnaporthe grisea(hebert)barr(无性世代：Pyricularia grisea(cook)Sacc)引起的稻瘟病是水稻三大病害之一，培育和种植抗病品种是控制这一病害最经济有效的途径。然而生产实践表明应用常规方法进行稻瘟病持久抗性育种十分困难，而且已经育成的抗病品种往往只能维持3～5年。随着分子标记技术的迅速发展，目前已成为植物抗病基因研究的有力手段，在水稻抗稻瘟病基因研究方面已取得重要进展。 沈农606是沈阳农业大学稻作研究所选育成的超级稻品种，目前已在北方广泛种植，其田间表现对北方稻瘟病菌株具有很好的抗性。本研究选用抗稻瘟病品种沈农606与普感品种丽江新团黑谷配制组合，对F_2群体(527株)接种稻瘟病菌ZA1生理小种，鉴定结果表明F_2群体中抗病和感病的分离比为399∶128，经卡方检验表明，粳稻沈农606对ZA1小种的抗病性表现为由一对基因控制的显性遗传。 利用84对SSR引物对沈农606和丽江新团黑谷进行多态性检测，结果有35对引物在抗、感亲本间扩增出了多态性，多态性表现率为44.9％。利用这35对引物对F_2群体(110株)进行扩增，结果筛选出两个与沈农606中抗稻瘟病基因相连锁的引物RM250和RM208。并初步确定沈农606对稻瘟病菌ZA1小种的抗病基因位于第2号染色体上，与SSR标记RM250和RM208的遗传距离分别为4.6和6.1cM。
Rice blast disease , caused by Magnaporthe grisea (hebert) barr, is one of the three major disease in rice. Utilization of genetic resistance is the most effective and environment friendly strategy for the disease control. However, breeding for stable resistance to blast has been extremely difficult. Although some cultivars with durable resistance has been recognized , the resistance of most cultivars is still short-lived. The molecular marker technology is a certain approach to overcome the difficulty, because the presence of markers tightly linked to resistance genes will allow selection and maintenance of the desirable resistance genotypes inbreeding process.SN606 is the super-rice cultivars cultivated by Shenyang Agricultural University Rice Research Institute. Recently it has been growing extensively in north of China, and the cultivars expresses a high resistance to the north rice blast races. In order to effectively using its broad spectrum resistance, two rice varieties, blast resistant SN606 and blast susceptible LTH, have been crossed. The race ZA1 of Magnaporthe grisea (hebert) barr have been used to inoculate the two parents, F_1, F_2 population in the conservatory. The results show that the segregation ratio of resistance and susceptible to ZA1 was 399:128 in F_2 population. The X~2 test shows that the genetic mechanism of resistance of ZA1 blast race is controlled by single dominant blast-resistance gene.The polymorphism between parents was examined by 84 pairs of primers of SSR. The results show that among the 84 pairs of primers, there are 35 pairs of primers displaying the polymorphism between two parents, the polymorphism rate is 44.9%. The PCR was carried out with DNA of F_2 population under the 35 pairs of primers. The results reveal that two primer RM250 and RM208 is linked with the blast resistance gene of ZA1 of SN606, and preliminary mapped in chromosome 2 of rice. The possible linkage value was 4.6 and 6.1cM with marker RM250 and RM208.
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