论文标题:三个木薯优良品种的组织培养技术研究
Study on Tissue Culture Techniques in Three Varieties of Cassava (Manihot Esculenta Crantz)
论文作者 李斌
论文导师 杨美纯,论文学位 硕士,论文专业 作物栽培学与耕作学
论文单位 广西大学,点击次数 113,论文页数 49页File Size3690k
2003-05-01论文网 http://www.lw23.com/lunwen_212281757/ 木薯,多效唑,组织培养,快速繁殖
cassava;paclobutrazol;tissue culture;rapid propagation
本试验选用“绿宇特”、“GR911”、“辐选01”三个木薯优良品种带腋芽的茎段作为外植体进行培养，分别在无性培养系建立，芽的增殖，生根和移栽四个阶段进行了初步研究，得出以下结果： 1、无性培养系建立阶段 在冬季、春季、夏季三个季节选取三个木薯品种的茎段进行培养，对污染率、腋芽萌芽率和生长状况进行了比较。试验表明，木薯最适宜选取春季生长的茎段进行培养，幼嫩的外植体灭菌效果好。MS＋BA1.0—1.5mg／L培养基较适合于腋芽生长。 2、芽增殖阶段 在MS培养基中附加一定浓度的BA(0.5—2.5mg／L)，可诱导三个品种茎段上的腋芽形成丛芽，其中MS＋BA2.0mg／L较适合“绿宇特”形成丛芽，MS＋BA1.5mg／L较适合“GR911”、“辐选01”，形成丛芽。“辐选01”表现出与前两个品种不同的特性，在MS＋BA1.5—2.0mg／L＋NAA0.05mg／L培养基中增殖培养，可从丛芽基部瘤状愈伤组织分化出芽，BA与NAA配合使用，对愈伤组织分化芽的效果优于单一使用BA。 3、生根阶段 NAA诱导三个品种幼苗生根的效果优于IBA。用多效唑诱导生根，使幼苗矮壮，植株叶片较绿，根粗，达到壮苗效果，有利于提高幼苗的移栽成活率。较适合诱导三个品种幼苗生根的培养基为1／2改良MS＋PP_(333)0.2—0.5mg／L或1／2改良MS＋NAA0.1mg／L。，4、移栽阶段 温度和水分对三个品种组培苗移栽成活率影响较大。温度低。基质水 分多，易造成组培苗死亡。3月份的移栽成活率为 70％左右。
Stems with axillary buds of cassava in three varieties- " Luyute" , "GR911"and "Fuxuan 01" were selected for in vitro culture. Four stages of primary research were processed, that is, establishment of clone system, multiplication of shoot, rooting and transplantation. The results are shown as below:1、 Stage of establishment of clone systemThe stems of three varieties were selected to culture in winter, spring and summer, to compare their contamination rate, sprouting rate and growth condition of buds. It was best for cassava to culture in spring. The effect of sterilization was perfect when the explants were immature stems. MS medium supplemented with 1.0-1.5 mg/L BA were fit to induce sprouting. 2、Stage of multiplication of shootMS medium supplemented with 0.5-2.5mg/L BA could induce multiple shoots in three varieties. MS medium supplemented with 2.0mg/L BA was fit for "Luyute", while 1.5mg/LBA was fit for "GR911"and"Fuxuan 01". "Fuxuan 01" showed some properties different from the other two varieties. When subcultured in MS medium supplemented with 1.5-2.0mg/L BA and 0.05 mg/L NAA, shoot could be regenerated from warty-like callus that formed on the bottom of multiple shoots. So the combination of BA and NAA would play a better role in inducing shoots from callus than BA alone. 3、Stage of rootingThe rooting effect which induced by NAA was superior to that of IBA inthree varieties. When rooting induced by paclobutrazol, shoots were short and strong, leaves were greener and roots were thick., thus promoted the survival rate of transplantation. For all the three varieties, the modified 1/2 MS medium supplemented with 0.2-0.5 mg/L paclobutrazo or modified 1/2 MS medium supplemented with 0.1 mg/L NAA were suitable for rooting.4、Stage of transplantationTemperature and moisture affect the survival rate of transplantation. Low temperatuer and high degree of moisture would lead to the death of plantlet. The survival rate of transplantation in three varieties were 70% in March.
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