论文标题:口服福尔马林对饲喂玉米秸秆日粮绵羊瘤胃消化代谢影响的比较研究
Comparative Study on the Effects of Drinking Formalin on Digestion and Metabolism of Rumen in Sheep in Corn Stalk-based Diet
论文作者
论文导师 雒秋江,论文学位 硕士,论文专业 动物营养与饲料科学
论文单位 新疆农业大学,点击次数 225,论文页数 58页File Size1956K
2006-06-01论文网 http://www.lw23.com/lunwen_2677702/
formalin; sheep; rumen; digestion
本实验选取4只体重约35Kg、2岁龄装置了永久性瘤胃瘘管和十二指肠瘘管的新疆美利奴羯羊,采用4×4拉丁方设计,研究口服福尔马林对饲喂玉米秸秆日粮绵羊瘤胃消化代谢的影响。结果表明,给绵羊分别喂给0(对照)、0.5、1.0、1.5ml/羊/日福尔马林,各处理组羊的玉米秸秆采食量分别为637.1±32.6、692.9±34.7、744.8±65.3和760.3±78.5克/羊/天,瘤胃液氨态氮平均浓度分别为26.1±2.0、24.4±2.1、23.6±2.9和21.8±2.0 mg/100ml,乙酸平均浓度分别为64.92±3.81、65.21±3.38、66.17±3.25和66.46±3.35 mmol/L,丙酸平均浓度分别为15.29±0.64、16.21±0.34、17.61±0.98和17.98±1.21 mmol/L ,丁酸平均浓度分别为10.43±0.83、10.64±0.70、11.30±0.83和11.52±0.99 mmol/L,总VFA平均浓度分别为90.64±5.06、92.06±3.81、94.66±4.14和95.95±5.86mmol/L,原虫平均数量分别为71.7±1.7、52.9±13.7、47.5±15.7和40.3±18.5(×104/ml)。前胃有机物消失量分别为510.1±63.9、594.1±83.8、628.3±62.9和634.4±76.4克/羊/天,前胃纤维素消化率分别为53.1±3.8、61.1±3.2、66.2±17.3和64.5±7.2%,到达十二指肠的粗蛋白量分别为82.3±6.0、87.3±3.8、96.1±6.9和97.8±3.6克/羊/天、微生物蛋白量分别为64.8±2.5、64.6±9.5、61.3±7.8和60.2±7.1克/羊/天、过瘤胃饲料蛋白量分别为16.1±4.3、20.5±11.0、32.9±13.2和35.6±4.9克/羊/天。实验表明,给绵羊口服福尔马林可提高动物对玉米秸秆的采食量,降低瘤胃液氨态氮的浓度,具有增加乙酸和丙酸的浓度从而增加总挥发性脂肪酸浓度的趋势,增加前胃有机物的消失量和纤维素的消化量,增加到达小肠的粗蛋白总量和过瘤胃饲料蛋白量,并且有减少到达小肠的微生物蛋白量的趋势。福尔马林以上这些作用均随剂量的增加而提高,然而,从总体看,绵羊每天口服1ml福尔马林(分两次喂给时)比较适于改善瘤胃消化代谢状况。
4 of Xinjiang Merino sheep, with body weight of 35 Kg and with the canula in rumen and duodenum, were designed as 4×4 Latin squares, to study the effect of drinking formalin on the digestion and metabolism of rumen. The results showed that when the animals were orally given formalin with 0, 0.5, 1.0 or 1.5ml/day respectively, the average concentrations (AC) of ammonia nitrogen in rumen were 26.1±2.0, 24.4±2.1, 23.6±2.9 and 21.8±2.0 mg/100ml g/sheep/d, the AC of acetic acid were 64.92±3.81,65.21±3.38,66.17±3.25and 66.46±3.35 mmol/L g/sheep/d respectively, the AC of propionic acid were 15.29±0.64,16.21±0.34,17.61±0.98and 17.98±1.21 mmol/L respectively, the AC of butyric acid were 10.43±0.83,10.64±0.70,11.30±0.83and 11.52±0.99 mmol/L respectively, the AC of total VFA were 90.64±5.06,92.06±3.81,94.66±4.14and 95.95±5.86 mmol/L g/sheep/d respectively, and the average amounts of protozoan in rumen were 71.7±1.7,52.9±13.7,47.5±15.7 and 40.3±18.5(×104/ml) g/sheep/d。The OM disappearance from rumen was 510.1±63.9, 594.1±83.8, 628.3±62.9 and 634.4±76.4 g/sheep/d respectively, the cellulose digestibility from rumen was 53.1±3.8, 61.1±3.2, 66.2±17.3 and 64.5±7.2% respectively,the crude protein arriving duodenum was 82.3±6.0, 87.3±3.8, 96.1±6.9 and 97.8±3.6g/sheep/d respectively, the microbial protein arriving duodenum was 64.8±2.5,64.6±9.5,61.3±7.8 and 60.2±7.1g/sheep/d respectively, and the bypass protein was 16.1±4.3, 20.5±11.0, 32.9±13.2 and 35.6±4.9g/sheep/d respectively. It is concluded that drinking formalin would increase the intake of corn straw by sheep, decrease the concentrations of the ammonia nitrogen, has tendency of increase the acetic acid and propionic acid, therefore the total VFAs in rumen, and increase the amount of protozoan. Drinking formalin would increase the OM disappearance from rumen, the crude protein and bypass protein arriving duodenum, with the tendency of decreasing microbial protein arriving duodenum. It is considered that drinking 1 ml of formalin (twice a day) is a suitable dose to improve the digestion and metabolism of rumen of sheep.
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