论文标题:广西毛南族人群CSF1PO，TPOX，TH01基因座遗传多态性研究
Study on Polymorphisms of 3 Str Locus of CSFIPO, TPOX and THO1 in MaoNan Ethnic Group in Guangxi Province
论文作者 滕少康
论文导师 劳明,论文学位 硕士,论文专业 人体解剖与组织胚胎学
论文单位 广西医科大学,点击次数 38,论文页数 40页File Size3110k
2005-05-01论文网 http://www.lw23.com/lunwen_578395757/ 短串联重复序列;PCR;基因频率;遗传距离; 毛南族;系统发生分析
Short Tandem Repeat;PCR;Gene frequency;Genetic Distance; Maonan Ethnic Group;phylogenetic analysis
目的:获得广西毛南族人群3个STR基因座: CSF1PO,TPOX,TH01的群体遗传学资料。探讨广西毛南族人群的起源。材料与方法:200份血样采自广西环江毛南族自治县,检测对象均为毛南族的健康无关个体。应用Chelex-100提取法提取DNA,AmpF?STR IdentifilerTM荧光标记复合PCR扩增技术扩增3个STR基因座,ABI Prism? 3100型遗传分析仪检测扩增产物。统计方法: SPSS-χ2检验;系统进化分析软件Mega Version 2.1构建系统进化树。结果:TPOX检测出5种等位基因,14种基因型。TH01检测出6种等位基因,19种基因型。CSF1PO检测出7种等位基因,21种基因型。以上3位点的基因型分布均符合Hardy-Weinberg平衡定律(P>0.05)。广西毛南族TPOX,TH01,CSF1PO位点的杂合度分别是0.625、0.735、0.695;个体识别力分别是0.7900、0.8609、0.8937;非父排除率分别为0.4590、0.5933、0.6735。遗传距离计算结果:广西壮族(0.0278)→广西水族(0.0327)→广西苗族(0.2173) →海南黎族(0.2208)→广西京族(0.2316)→云南傣族(0.2336) →云南布依族(0.2367)→广西汉族(0.2459)→新疆哈萨克族(0.5202)。系统进化分析结果:广西毛南族与广西壮族、广西水族、广西苗族、海南黎族、广西京族、云南傣族、云南布依族集为一簇,广西汉族与新疆哈萨克族聚成另外一簇。结论:广西毛南族与广西壮族、广西水族遗传差异最小,亲缘关系最近;本研究结果从常染色体STR多态性角度佐证了广西毛南族起源于古代百越民族。广西毛南族3个STR基因座属于高度多态性遗传标记,所得到的群体遗传学数据能为法医学个体识别和亲权鉴定提供概率计算依据。
Objective: This study was carried out to investigate the distribution of threeshort tandem repeats (STR) loci in Maonan ethnic group in GuangXi province andexplored the origins of Maonan ethnic group. Materials and methods: Bloodspecimens were collected from 200 healthy unrelated Maonan individuals inGuangXi province, DNA was extracted by using method of Chelex-100 extracting.Three STR locus in Maonan ethnic group were screened by using AmpF?STRIdentifilerTM PCR Amplification Kit and 3100 Genetic Analyzer. Statisticsanalysis:SPSS-χ2 examination and phylogenetic analysis. Results: There were 5alleles and 14 genotypes in TPOX locus, 6 alleles and 19 genotypes in TH01 locus,7 alleles and 21 genotypes in CSF1PO locus. The allele distributions of the lociwere in good agreement with Hardy-Weinberg equilibrium. The heterozygosity(H)of the 3 locus were 0.625、0.735、0.695 respectively; the discrimination powersuccessively were(DP) 0.7900、0.8609、0.8937 and the exclusion probability ofpaternity exclusion(EP) were 0.4590、0.5933、0.6735 in turn. The result ofmeasuring Genetic Distance showed: Zhuang in Guangxi province(0.0278)→Shuiin Guangxi province(0.0327)→Miao in Guangxi province (0.2173) →LiinHainan province(0.2208)→Jing in Guangxi province(0.2316)→Dai in Yunnanprovince (0.2336) →Buyi in Yunnan province(0.2367)→Han in Guangxiprovince(0.2459)→Hasake in Xinjiang province(0.5202). The result ofphylogenetic analysis showed: Maonan, Zhuang , Shui, Miao in Guangxi province,Li in Hainan province, Jing in Guangxi province, Dai in Yunnan province, BuyiinYunnan province became one cluster. Han in Guangxi province and Hasake inXinjiang province was another cluster. Conclusions: The relationship betweenMaonan, Zhuang and Shui in Guangxi province is the closest .Combinded withresearch results of ethnology,linguistics and Y-SNP, Maonan in Guangxi provincepossibly originated from ancient Baiyue minority. The datas obtained can be alsoused in population forensic application.
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