论文标题:YR-1对肿瘤化疗减毒作用的实验研究
Ginsenoside YR-1 Attenuated Chemotherapy-induced Toxicity
论文作者
论文导师 卿晨,论文学位 硕士,论文专业 药理学
论文单位 昆明医学院,点击次数 121,论文页数 53页File Size9428K
2006-05-01论文网 http://www.lw23.com/lunwen_59338202/
YR-1; chemotherapy; attenuation; macrophage; bone marrow depression (BMD)
目的：研究人参皂苷类化合物YR-1对毒性剂量的化疗药顺铂DDP和紫杉醇TAX治疗小鼠肝癌H22疗效及对荷H22小鼠胸腺和脾脏的影响。研究YR-1对DDP和TAX所致荷H22小鼠巨噬细胞(Mφ)吞噬功能抑制和骨髓抑制的影响。方法：以小鼠移植性肝癌H22为模型，采用称重法检测YR-1不同剂量(0.5、1.5、4.5mg／kg)经口给药对毒性剂量的DDP(2mg／kg)和TAX(20mg／kg)抑瘤作用及对荷瘤小鼠胸腺和脾脏指数的影响；采用中性红吞噬法和骨髓涂片法检测YR-1对DDP和TAX所致荷H22小鼠Mφ吞噬功能抑制和骨髓抑制的影响。结果：YR-1对DDP治疗小鼠肝癌H22的疗效无降低作用，与同等剂量的DDP相同；YR-1低、中剂量对TAX治疗小鼠肝癌H22的疗效无降低作用，与同等剂量的TAX相同，YR-1高剂量对TAX治疗小鼠肝癌H22的疗效有增强作用，高于同等剂量的TAX；YR-1各剂量组对DDP、TAX所致荷瘤小鼠Mφ的吞噬功能均有明显的增强作用(与相应单一化疗药组比，P＜0.01)；YR-1各剂量均能提高DDP、TAX所致荷瘤小鼠骨髓抑制的增生级别，并呈现出一定的量效关系。结论：在不降低DDP和TAX疗效的情况下，YR-1对化疗药物所致荷瘤动物免疫功能的降低和骨髓抑制具有明显的拮抗作用，提示YR-1对化疗所致的免疫功能损伤和骨髓损伤具有保护作用。
OBJECTIVE: To study effects of YR-1 on the toxicity of immune and bone marrow induced by chemotherapy drugs DDP or TAX at the toxicity dosage in mice bearing H22 (Hepatoma 22). METHODS: The effects of YR-1 on tumor growth and thymus /spleen of mice bearing H22 were measured by weighing. The effects of YR-1 on the phagocytizing function of macrophage and bone marrow depression of mice bearing H22 induced by chemotherapy drugs DDP or TAX at the toxicity dosage were determined by phagocytizing neutral red test and bone marrow film preparation. RESULTS: The results show that YR-1 did not reduce and even enhance the inhibitory effect of DDP or TAX on H22 of mice. In mice bearing H22, YR-1 significantly enhanced the phagocytic function of macrophage inhibited by DDP or TAX and upgraded the level of bone marrow hyperplasia depressed by DDP or TAX. CONCLUSION: YR-1 can attenuate the toxicity of immune function and bone marrow induced by chemotherapy drugs DDP and TAX in condition of unaffecting the efficacy, suggest YR-1 possesses the protective effect on the damage of immune function and bone marrow indued by chemotherapy drugs.
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