论文标题:有氧运动对大鼠肝脏胆固醇流出调节蛋白影响的研究
Effects of Aerobic Exercise on Cholesterol Efflux Regulatory Protein of Liver
论文作者
论文导师 王国祥,论文学位 硕士,论文专业 运动人体科学
论文单位 苏州大学,点击次数 175,论文页数 56页File Size934K
2007-03-01论文网 http://www.lw23.com/lunwen_6302837/
aerobic exercise ;;liver;; cholesterol efflux;; ABCA1mRNA ;;SR-BI mRNA
目的: 胆固醇是机体不可缺少的成分,但机体内积累过多的胆固醇会导致高脂血症等多种疾病的发生。运动尤其是有氧运动对高脂血症等疾病具有预防和治疗作用,可以在一定程度上优化血浆脂蛋白的组成。ABCA1、SR-BI是细胞胆固醇流出的调节蛋白。为了进一步揭示运动对胆固醇流出机制,本文采用了有氧运动方式对大鼠肝脏胆固醇流出调节蛋白ABCA1、SR-BI基因表达的影响进行了实验研究。 方法: SD雄性大鼠,52只,体重190g-210g。随机分为:N组(n=20)和H组(n=32)。6周高脂高胆固醇诱导H组大鼠致高胆固醇血症,从中选出高胆固醇大鼠27只,随机分为HS组(n=12)和NE(n=15)组;与此同时,N组分为NS组(n=10)和NE组(n=10)。运动组大鼠进行6周无负重的游泳运动,每周6天:第1、2周每天45min,第3、4周每天60min,第5、6周每天90min。最后一次运动后,所有大鼠禁食12h后,水合氯醛麻醉,腹主动脉取血,制备血浆,测定TC、TG、HDL-C、LDL-C,并迅速取肝脏经生理盐水清洗,放入预先经焦炭二乙酯处理过的管中,并放入冷冻冰盒,马上转入-80℃的冰箱中,待测肝脏ABCA1、SR-BI基因表达。 结果: (1)6周高脂膳食,大鼠体重和Lee Index:H组大鼠体重和Lee Index增加,血浆TC明显升高。6周游泳运动后,大鼠体重增加幅度下降,Lee Index降低。 (2)6周游泳运动后,大鼠血浆TC、TG显著下降;LDL-C水平HS组高于NS组,HE组高于NE组,NE组低于NS组,HE组低于HS组;HDL-C水平H组显著低于N组,运动组显著高于对照组;AI、TC/HDL-C、LDL-C/HDL-C运动组均低于非运动组,H组均大于N组。 (3)6周游泳运动后大鼠肝脏ABCA1mRNA和SR-BI mRNA:运动组ABCA1mRNA高于非运动组,H组高于N组。肝脏SR-BI mRNA ,HS组明显低于NS组,HE组低于NE组,NE组高于NS组,HE组显著高于HS组。 结论: 有规律的有氧运动可以上调细胞胆固醇调节蛋白ABCA1和SR-BI的基因表达,进而提高细胞胆固醇的转运与排泄,在一定程度上对抗高脂膳食导致的脂质代谢紊乱,改善血浆脂质水平。
Objective: Cholesterol is important in body. But, it will lead to many diseases such as hyperlipoidemia for overmuch cholesterol. Aerobic exercise can improve plasma lipid level effectually. The mechanism of aerobic exercise on cholesterol efflux regulation protein sisn’t clear. This research studied the effect of aerobic exercise on rats’liver ABCA1mRNA and SR-BI mRNA. Method: 52 male SD rats (190g-210g), were randomly divided into two groups: normal group (N, n=20) and high group (H, n=32). The H group was fed high-lipid-diet for 6 weeks to build hyperlipoidemia model. 27 rats were chosen out from H group, then divided into two groups: HS (n=12) and HE (n=15). The N group was divided into two groups too: NS (N=10) and NE (n=10). The HE group and NE group had swim for 6 weeks, 6 days per week: the 1st and 2nd week for 45 min, the 3rd and 4th for 40 min, the 5th and 6th for 90 min. After 6 weeks, the last swim, all rats were forbidden to eat for 12h.Then, to kill them, get plasma to determine TC、TG、HDL-C and LDL-C , get liver to determine ABCA1mRNA and SR-BI mRNA. Results: (1) After 6 weeks high-lipid-diet, The weight and Lee Index increased obviously. The plasma TC level was increased. After 6 weeks swim, the rats weight increased slowly, Lee Index decreased. (2) After 6 weeks swim , TC、TG and LDL-C are obviously decreased. But HDL-C increased. AI、TC/HDL-C and LDL-C/HDL-C all decreased. (3) After 6 weeks swim, the exercise groups’ABCA1mRNA are higher than the non-exercise. HE and HS are higher than NE and NS.HS group’SR-BI mRNA of the liver is lower than NS, HE is lower than NS, HE is higher than HS. Conclusion: Aerobic exercise can increase ABCA1mRNA and SR-BI mRNA, and increase the cholesterol transport and excrete. So, aerobic exercise can oppose against the tendency that plasma lipid metabolic disorder for high-lipid-diet, which improve plasma lipid level.
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