论文标题:MDV与REV感染肉鸡时的相互作用和MDV重组野毒株的研究
Studies on Interaction of Marek"s Disease Virus and Reticuloendotheliosis Virus in Co-infected Broilers and Research on MDV Recombinant Field Strain
论文作者
论文导师 崔治中,论文学位 硕士,论文专业 预防兽医学
论文单位 山东农业大学,点击次数 561,论文页数 102页File Size939K
2006-06-06论文网 http://www.lw23.com/lunwen_632997/
Marek’s disease virus reticuloendotheliosis virus; co-infection; interaction; viremia; antibody titers
为了研究鸡马立克氏病病毒(MDV)和网状内皮增生病病毒(REV)共感染时的相互作用,分别在REV母源抗体阳性(REV-Ab+)和阴性(REV-Abˉ)及经MDV疫苗CVI988株免疫和不免疫的商品代肉鸡,比较了二种病毒在病毒血症水平和特异抗体效价上的相互影响。结果表明,在经MDV弱毒疫苗CVI988/Rispens株免疫的鸡,如果有REV母源抗体预防REV病毒血症,在接种强毒MDV后, 5周龄时都检不到强毒MDV病毒血症。但在REV-Abˉ鸡,在显示REV病毒血症的同时,也显示强毒MDV的病毒血症。而在未经CVI988/Rispens免疫的鸡,虽然在有REV-Ab+的鸡(133.3±326.6)的REV病毒血症比REV-Abˉ的鸡(1808.3±1413.7)低的多,在MDV病毒血症水平上没有任何区别。但是,却比经MDV弱毒疫苗CVI988/Rispens株免疫但REV-Abˉ的鸡高的多(41.67±33.7)。这表明,REV共感染时MDV病毒血症水平的影响主要是通过降低CVI988/Rispens疫苗的免疫效力发挥作用的。同是在REV-Abˉ鸡,强毒MDV病毒血症水平的高低并不影响攻REV后的病毒血症水平。但是,在都有REV母源抗体的鸡,如果不使用CVI988/Rispens疫苗预防MDV病毒血症,MDV共感染造成的某种免疫抑制,使REV母源抗体阳性的鸡不再能完全有效地抵抗REV感染造成的病毒血症。 在用CVI988/Rispense免疫的鸡,其诱发的MDV特异性抗体效价受REV病毒血症的影响很大,特别是在有REV母源抗体的1和7组,在接种REV后均不会有病毒血症,其MDV特异性抗体效价分别是3.8±0.8 (Log10)和4.2±1.0 (Log10),显著高于没有REV母源抗体而会产生REV病毒血症的2组和8组的1.8±1.0(Log10)和2.3±1.6(Log10)。结果表明,REV感染将显著地抑制CVI988/Rispense免疫诱发的抗体水平。 在未经CVI988/Rispense免疫的鸡,分别有REV母源抗体或无REV母源抗体。虽然都在1日龄接种了REV,然而REV病毒血症水平可能差异很大。经测定的3组和4组的结果表明,虽然二组REV病毒血症水平差异很大(133.3±326.6 PFU/ml对1808.3±1413.7 PFU/ml),但二组间由横
To understand the interaction between Marek’s disease viruses (MDV) and reticuloendotheliosis viruses (REV) in co-infected chickens, their mutual influences on vriremia levels and specific antibody titiers were studied in REV maternal antibody positive (REV-ab+) or negative (REV-ab-) commercial broilers vaccinated with or without MDV vaccine CVI988. The results indicated that,in chickens of vaccinated with CVI988/Rispense,if REV-ab+ to prevent REV viremia, after inoculated virulent MDV, there can′t detected virulent MDV viremia at 5 weeks.But in chickens with REV maternal antibody negative ,both REV and MDV viremia can be detected.In chickens unvaccinated CVI988/Rispense, the REV viremia of REV-ab+ (133.3±326.6) was lower than REV-ab- chickens(1808.3±1413.7), and no difference in MDV viremia level. but their MDV viremia level were higher than vaccinated CVI988/Rispense and REV-ab-(41.67±33.7). This indicated that REV influence MDV viremia through reducing the immune effect. In chickens of REV-ab- , the level of virulent MDV viremia didn′t influence the REV viremia .However, all the REV-ab+ chickens, if were unvaccinated CVI988/Rispense to prevent MDV viremia. MDV mutual influence producted inhibitory effected on chicken′s immune functions, the REV-ab+ chickens can not completely and effectively prevent REV viremia. In chickens vaccinated with CVI988/Rispense,REV viremia influencted MDV specific antibody titers. Especially in 1 and 7 group of REV-ab+ chickens, no REV viremia were detected after inoculated REV. MDV specific antibody titers were 3.8±0.8 (Log10) and 4.2±1.0 (Log10),strikingly highly than 2 and 8 group of REV-ab- chickens 1.8±1.0(Log10)和2.3±1.6(Log10) , REV viremia were detected. The result indicated, REV mutual strikingly producted inhibitory antibody level induced by CVI988/Rispense. In chickens of unvaccinated CVI988/Rispense. Respectively REV-ab+ and REV-ab-. Althrough inoculated at 1 day age, their REV viremia level very
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