论文标题:糖基化兔血小板体内外功能研究
The Function of Galactosylation Rabbit Platelet in Vivo and in Vitro
论文作者
论文导师 陈宝安,论文学位 硕士,论文专业 临床检验诊断学
论文单位 东南大学,点击次数 85,论文页数 32页File Size4522K
2006-06-05论文网 http://www.lw23.com/lunwen_637262037/
Galactosylation;UDP-Gal;cold-stored platelet;rabbit model;bleeding time; percentage plate recovery
目的:研究了糖基化兔血小板冷藏保存后的体内外功能,探讨了以尿苷二磷酸半乳糖(UDP-Gal)为添加剂的血小板冷藏保存方法。 方法:采集兔心脏血,按常规方法制备浓缩血小板悬液(PCs),在悬液中添加UDP-Gal,放4℃冰箱储存10天。尔后观察血小板(PLT)数量﹑血小板平均体积(MPV)、血小板体积分布宽度(PDW)﹑血小板聚集功能(PagT)﹑血小板促凝活性实验(PF3aT和APCT)和血小板凋亡(Apoptosis)的变化;将冷藏兔血小板用Cr51标记后,检测输入兔体内后的生存时间;输入兔血小板减少症模型体内,检测输注后1h和24h兔耳出血时间,和输后血小板回收率(PPR)。 结果:加入UDP-Gal冷藏保存10天后的PC的PLT、MPV、PDW、血小板促凝血活性与新鲜PC相比均无显著性变化,p﹥0.05;而冷藏对照PC的PLT明显减少,MPV、PDW显著增大,并且血小板促凝血活性减低,与新鲜PCs相比p值均小于0.01;加入UDP-Gal的PC冷藏保存10天后的血小板凋亡率与新鲜PC相比有所增高,p﹤0.05,但明显低于冷藏对照组,p﹤0.01,其血小板聚集率(诱聚剂为阳离子没食子酸丙酯,C-PG)减低,但仍能保持在新鲜血小板的50%以上;添加UDP-Gal冷藏保存10天的血小板与新鲜的血小板在体内的生存时间相近,p﹥0.05,输注兔体内72h时的血小板存活率在新鲜对照组、UDP-Gal冷藏保存组和冷藏对照组分别为57.5%±7.2%、50.3%±6.3%和0.1%±0.5%。糖基化冷藏兔血小板输注后可明显缩短兔耳出血时间,与新鲜兔血小板组相比无统计学差异,p >0.05;而冷藏兔血小板对照组输注前后的兔耳出血时间无显著变化, p﹤0.01;新鲜血小板组﹑糖基化冷藏血小板组﹑冷藏血小板组输注后1h和24h的PPR分别是66.1%±0.5%﹑47.8%±0.6%,60.9%±0.3%﹑41.6%±0.4%,47.7%±0.5%﹑11.4%±0.5%;糖基化冷藏兔血小板组与新鲜兔血小板组相比,其输注后PPR无统计学差异,p >0.05,糖基化冷藏兔血小板组和新鲜兔血小板组的PPR均明显高于冷藏血小板对照组,p<0.01。 结论:糖基化能明显改善冷藏兔血小板的体内和体外功能,延长冷藏兔血小板保存时间。
Objective: This study was aimed to investigate the function of galactosylation cooled rabbit platelet in vivo and in vitro. Methods: We collected rabbit heart blood, prepared concentrated platelet suspension in a normal way to which we add UDP-Gal ,and then stored them for ten days in 4℃refrigerator. Thereafter,platelet count,mean platelet volume ,platelet distributing width ,platelet aggregation function,platelet activity to urge coagulation includingPF3aT and APCT and apoptosis were determined .Meanwhile,survival time in vivo was tested after cold-stored rabbit platelets labeled with Cr51 were transfused into rabbits; Rabbit ear bleeding time ,APCT, percentage plate recovery(PPR) were determined in the first and the twenty-fourth hour after they were transfused into model of rabbit thrombocytopenia. Results: There is not significant difference for PLT count、MPV、PDW、PF3aT and APCT between UDP-Gal cold-stored platelet group and fresh platelet group(p﹥0.05).On the contrary,platelet count decrease significantly ,MPV,PDW jump and PF3aT and APCT go down in cold contral group compared with fresh platelet group(p﹤0.01).Apoptosis increase in UDP-Gal cold-stored platelet group compared with fresh platelet group(p﹤0.05),but is significantly lower than that in cold contral group(p﹤0.01). Although PagT(inducing reagent:C-PG) decrease,it can still be above 50% of fresh platelet;Survival time in rabbit vivo is close between UDP-Gal cold-stored platelet group and fresh platelet group(p﹤0.05). Survival rate in the seventy-two hour after transfusion in fresh platelet group, UDP-Gal cold-stored platelet group and cold contral group respectively is 57.5%±7.2%,50.3%±6.3%和0.1%±0.5%; Rabbit ear bleeding time was significantly shortened after transfusion of galactosylation cooled rabbit platelet,and there was not significant difference between UDP-Gal cold-stored platelet group and fresh platelet group(p﹥0.05).On the contrary,it had less change in cold control group(p﹤0.01);PPR was respectively 66.1%±0.5%,47.8%±0.6%;60.9%±0.3%,41.6%±0.4%;47.7%±0.5%,11.4%±0.5% in fresh platelet group, UDP-Gal cold-stored platelet group and cold control group. PPR after transfusion of galactosylation cooled rabbit platelet has no statistical difference compared with that of fresh platelet group(p﹥0.05). Conclusions:Galactosylation can effectively enhance the function of cooled platelet in vivo and in vitro, and prolong the storage time of them.
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