论文标题:软坚散结药物防治增生性玻璃体视网膜病变的实验研究
Studies on Chemical Constituents and Hypoglycemic Effect of Stem of Gymnema Sylvestre
论文作者 武文忠
论文导师 王明芳,论文学位 博士,论文专业 中医眼科学
论文单位 成都中医药大学,点击次数 96,论文页数 74页File Size5680k
2005-04-01论文网 http://www.lw23.com/lunwen_660168797/ 增生性玻璃体视网膜病变(PVR);实验研究;化瘀散结片;软坚散结;肝细胞生长因子;结缔组织生长因子;透明质酸
proliferative vitreoretihopathy; experimentalize ; Huayusanjie tablet; softening and resolving hard mass; hepatocyte growth factor; connective tissue growth factor; hyaluronic acid
目的:通过检测肝细胞生长因子(HGF)、结缔组织生长因子(CTGF)、透明质酸(HA)的变化,观察化瘀散结片中的软坚散结药物对实验性增生性玻璃体视网膜病变(PVR)的影响,探讨软坚散结药物防治PVR的作用机制。 方法:采用“玻璃体内注入体外培养兔视网膜色素上皮(RPE)细胞”方法建立PVR动物模型,造模3天后给予化瘀散结片及软坚散结药物,连续灌胃60天,通过免疫组织化学方法和放射免疫法(RIA)检测HGF、CTGF和HA的表达。 结果:(1)化瘀散结片和软坚散结药物能明显降低HGF、CTGF在增生膜的表达(p<0.01或p<0.05),化瘀散结片治疗组优于软坚散结药物治疗组(p<0.05)。(2)化瘀散结片及软坚散结药物治疗组玻璃体HA含量明显低于模型对照组(p<0.01),化瘀散结片治疗组优于软坚散结药物治疗组(p<0.05)。(3)化瘀散结片及软坚散结药物高剂量治疗组总体效果优于低剂量治疗组(p<0.05)。 结论:实验性PVR的形成与HGF、CTGF表达增强和玻璃体内HA含量增高有关。化瘀散结片及软坚散结药物能减轻PVR的程度,作用机理与抑制HGF和CTGF在增生膜的表达、以及降低玻璃体内低分子量HA的含量有关。化瘀散结片全方总体效果优于软坚散结部分,表明化瘀散结片的另外部分(活血化瘀药物)与软坚散结部分有协同作用。化瘀散结片及软坚散结药物高剂量治疗组总体效果优于低剂量治疗组,表明化瘀散结片和软坚散结药物治疗PVR可能存在量效依赖关系。化瘀散结片及软坚散结药物还具有抗氧化、抑制自由基反应从而保护视网膜的重要作用。
Objective To observe the effects of the softening and resolving hard mass drugs in Huayusanjie tablet on hepatocyte growth factor(HGF), connective tissue growth factor(CTGE), hyaluronic acid(HA) in rabbit vitreous body of experimental proiiferative vitreoretinopathy(PVR) model, for exploring the mechanism of the softening and resolving hard mass drugs to prevent PVR. Methods Retinal pigment epithelium cells(RPEs) cultured in vitro were injected into rabbit vitreous body cavities respectively to establish experimental PVR model. Huayusanjie tablet or the softening and resolving hard mass drugs were fed respectively up to 60 days after the models had been established for 3 days. Then, RIA method and immunohistochemical method were used to detect the expression of HGF, CTGF and HA in vitreous body. Results 1; Huayusanjie tablet could significantly depress the compression of HGF and CTGF on proiiferative membrane, so did the softening and resolving hard mass drugs(P<0.01 or P<0.05) and the action of Huayusanjie tablet was more strong(P<0.05). 2. Compared with the model control group, there was significant lower HA content in the Huayusanjie tablet group or the softening and resolving hard mass drugs group(P<0.01); and compared with the softening and resolving hard mass drugs group, there was significant lower HA content in the Huayusanjie tablet group too(P<0.05); 3. Compared with the low dosage group, the action of preventing PVR was more strong in the high dosage group of Huayusanjie tablet or the softening and resolving hard mass drugs respectively. Occlusion There is certain relation between the
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