论文标题:不同佐剂类型的七种鸭疫里氏杆菌四价灭活苗免疫原性的比较研究
Comparisons Between the Immunogenicities of Seven Inactivated Riemerella Anatipestifer Tetravalent Vaccines with Different Adjuvants
论文作者 郭宇飞
论文导师 程安春；汪铭书,论文学位 硕士,论文专业 预防兽医学
论文单位 四川农业大学,点击次数 161,论文页数 42页File Size1931k
2002-06-01论文网 http://www.lw23.com/lunwen_74140047/ 鸭疫里氏杆菌;佐剂;灭活苗;免疫原性
Riemerella anatipestifer;adjuvant;vaccine;immunogenicity
本实验通过对供试鸭的攻毒保护试验、通过用ELISA方法对供试鸭特异性抗体消长规律的研究以及通过用~3H-TdR掺入法对供试鸭T细胞免疫水平的检测三个方面对所研制的七种不同佐剂类型的鸭疫里氏杆菌四价灭活苗的免疫原性进行了比较研究。 这七种疫苗在接种后一定时期内都可使鸭体产生完全的免疫保护，但它们在免疫力产生的速度和免疫力持续时间的长短方面有着明显的差异；蜂胶复合佐剂苗具有产生免疫保护速度快，免疫持续时间长的优点，接种后第3天即产生部分免疫保护力，第93天时仍具有完全保护力；油剂苗产生保护力的速度较慢，接种后第10天时开始表现出部分免疫保护，其完全保护力也可持续到接种后第93天；铝胶苗产生免疫保护的速度较慢，免疫持续期也较短，其开始产生部分免疫保护和开始产生完全保护的时间同油剂苗相当但其完全保护力只有约2—5周的持续时间。这七种疫苗在抗体产生速度方面以蜂胶苗较快，而油剂苗和铝胶苗免疫后抗体上升速度较慢；在抗体水平的维持能力方面，油剂苗较蜂胶苗和铝胶苗更强；在产生最高抗体水平方面，油剂苗和蜂胶苗能力相当且较铝胶苗为高；在加强免疫的效果方面，这些疫苗二次免疫所产生的抗体水平均较一次免疫更高，蜂胶苗和铝胶苗在第93天时的二免抗体水平已降至同一免相当，但此时油剂苗有着较一免更高的二免抗体水平。这七种疫苗免疫鸭的T细胞转化能力较非免疫鸭明显为高，但该作用只有约2周左右的持续期时间，此后便下降到与对照组相当的水平，二免可以使一免细胞免疫的水平增高和持续时间延长。通过检测同时还观察到：雏鸭的T淋巴细胞伴随机体的发育其转化能力不断提高。对这七种不同佐剂类型的鸭疫里氏杆菌四价灭活苗免疫原性的综合评价认为，蜂胶苗的免疫原性最好，油剂苗其次，铝胶苗最差，而这三类疫苗中又分别以蜂3、油3和铝3的免疫原性为最好。
Seven inactivated tetravalent vaccines of Riemerella anatipestifer (RA),of which three (1#,3#,7#) employed oil-emulsion,two(4#,6#) employed propolis,two(8#,10#) employed aluminum hydroxide as their basic adjuvants,were developed and compared by the methods as follows:challenges were performed by homologous virulent RA strains to measure their protective abilities;indirect enzyme-linked immunosorbent assay (ELISA) was used to monitor antibody production;lymphocyte transformation test (LTT) was performed to study the expression of cell-mediated immunity. The result indicated that effective levels of duckling protection,with different speeds and durations,could all be secured by administering one dose of these vaccines. Propolis vaccines could confer ducklings partial resistance to challenge as early as 3 days after immunization and absolute immunity still exist 93 days postvaccination. Oil-emulsion vaccines could confer ducklings partial resistance to challenge as early as 10 days after immunization and absolute immunity also exist 93 days postvaccination. Aluminum hydroxide vaccines could confer ducklings partial resistance to challenge as early as 10 days after vaccination but the duration of absolute immunity is only 2-5 weeks. ELISA showed that antibody responses induced by propolis vaccine were faster than that of oil-emulsion vaccines and aluminum hydroxide vaccines;antibody responses induced by oil-emulsion vaccines were longer in duration than that of propolis vaccines and aluminum hydroxide vaccines;the maximum antibody responses induced by oil-emulsion vaccines and propolis vaccines were higher than that of aluminum hydroxide vaccines;Antibody levels were all significantly boosted by revaccination of these vaccines and in addition to this,oil-emulsion vaccines showed longer antibody duration. Lymphocyte transformation test demonstrated that all these vaccines stimulated significantly higher levels of T cell-mediated immunity with a duration of 2 weeks or so than that of control after initial immunization and the levels and durations could both be boosted by revaccination. A phenomenon that the innate T cell-mediated immunity system of ducklings continued to develop as ducklings grew were also observed during the period of this test. Comprehensive comparison and analysis indicate that,of these seven vaccines,3#,6#,10# are better than other(s) in the same group;propolis vaccines are more excellent than oil-emulsion vaccines and aluminum hydroxide vaccines in immunogenicity.
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