论文标题:马拉色菌属生物学特性及其分子生物学鉴定的研究
Study on the Biologic Perperty and Molecular Biologic Detection
论文作者 徐琦
论文导师 许晏,论文学位 硕士,论文专业 免疫学
论文单位 新疆医科大学,点击次数 92,论文页数 40页File Size2560k
2004-05-22论文网 http://www.lw23.com/lunwen_78418957/ 马拉色菌属;生物学特性;分子生物学鉴定;前列腺炎
Malassezia; biologic perperties; molecular biologic detection;prostatitis
目的:对引起前列腺炎的可疑真菌的生物学特性研究,初步进行菌属鉴定,并从分子生物学角度探讨该菌种的鉴定。方法:常规方法取患者前列腺液,分离培养,得到可疑菌落。选择四种不同的培养基分别在不同温度条件下培养,选出最优培养条件;通过光学显微镜、电子显微镜观察菌落及菌体的形态学特点;通过芽管试验、碳水化合物发酵试验、碳水化合物同化试验、沙堡培养基生长试验、过氧化氢酶试验、七叶苷分解试验、吐温试验研究该菌的生理、生化学特征;应用PCR技术通过特异性的引物扩增得到菌属特异性的片段,最后测定扩增片段的核苷酸序列,从而进行菌种的分析、鉴定。结果:从临床获得的引起前列腺炎的可疑真菌为马拉色菌属;34℃,mDixon培养基、改良花斑癣培养基为该菌的最优培养条件;生物学特性的研究可将马拉色菌属成员部分鉴定至种;应用分子生物学技术可将马拉色菌属成员鉴定至种。结论:马拉色菌可引起深部感染,如前列腺炎,在临床前列腺炎的治疗中应引起足够重视;对该菌的鉴定不仅可依靠其特殊的生物学特性,还可以应用PCR技术对单个菌株进行分子生物学鉴定。
Objective: We have evaluated the fungi which was collected from clinic and was suspected to cause deep infection through the observation of biologic perperty and the molecular biology of these fungi. Methods: Take the prostata through the normal regulations method and study on the germ by the appearance characteristics. Selected the best cultivate condition by cultivated on four different media under different conditions; Observated morphology perperties; Studied on biology perperties by germ tube testing, Carbohydrate fermentation testing, Tween testing and Esculin dissociation; lysis testing. Amplificate the special fragment by using special primer and analysis the fragment. Results: The fungi which were collected from clinic and been suspected to cause deep infection were detected as Malassezia. Under 34 ℃, mDixon media is the best condition for these fungi. Conclusions: Malassezia can cause deep infection; medical treatment in clinical prostatitis should pay more attention; the detection of these fungi can not only through the observation of biologic perperty but also though the molecular biologic technique, PCR, which can detect the specific gene fragment of Malassezia.
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