论文标题:玫瑰香葡萄酚类物质动态变化及抗氧化活性
The Dynamic Changes of Polyphenols and Their Relationship with Antioxidant Capacity in Muscat Hamburg Grapevine
论文作者
论文导师 张元湖；田淑芬,论文学位 硕士,论文专业 植物学
论文单位 山东农业大学,点击次数 84,论文页数 70页File Size1310K
2007-06-10论文网 http://www.lw23.com/lunwen_79487737/
Muscat Hamburg;; phenolics;; flavonoids;; Proanthocyanidins(PAs);; Antioxidant Capacity;; Seedlessness Treatment
本文以玫瑰香葡萄为材料,研究了整个生长发育期内葡萄各个器官及无核处理葡萄的酚类物质及抗氧化能力变化,主要结果如下: 1从玫瑰香葡萄始花期开始,至浆果成熟期止,随着果实的发育,葡萄内各个器官的酚类物质含量呈现先下降后上升的趋势。叶片、茎、卷须中的酚类物质含量随着果实的发育逐渐降低,到果实生长发育期的快速生长期达到最低点,之后有所增加。而果实中,果皮自浆果生长期酚类物质含量开始下降,至浆果成熟期达最低点,之后有小幅度的上升趋势。原花色素一直呈降低趋势。果肉没有明显的变化,且酚类物质含量极低。 2各器官的抗氧化活性与其所含酚类物质含量呈显著正相关。ABTS+·法、DPPH法和O_2~-·作为测定抗氧化活性的方法是简便、有效、可行的。 3酚类物质在叶片中含量最高,其后依次为果皮>茎≥卷须>果肉。原花色素的分布为果皮>叶片>茎≥卷须>果肉。 4无核处理提高了叶片、茎和卷须内酚类物质的含量及其清除自由基的能力,而果皮和果肉中含量有所下降,相应的清除自由基的能力也低于对照。
Polyphenols and antioxidant capacity in different organs of Muscat Hamburg in the whole growth periods, and the effects of seedlessness treatment on polyphenols and antioxidant capacity of Muscat Hamburg were studied. The main results are as follows: 1. From the beginning of blooming period to the ripening period of Muscat Hamburg, the content of polyphenols in different organs decreased at first, and then increased with the development of the grape berry. The content of polyphenols in leaf, stem, and tentril decreased with the development of the grape berry, became the lowest in the fast growth stage of grape berry, and then increased to a certain degree. While the content of polyphenols in fruit skin decreased at the beginning of growing period of grape berry and became the lowest in the period of grape ripening period, and then increased to a certain degree. The content of PAs decreased in the whole growth period. There was no obvious change in grape pulp and the content of ployphenols was very low. 2. The remarkable positive correlation between the antioxidant capacities of grapevine organs and polyphenols was found. The method of using ABTS~+·, DPPH and O_2~-·scanvenging to measure the antioxidant capacities is convenient, efficient and feasible. 3. The contents of polyphenols in the leaf was the higheat and then the order was fruit skin>stem≥tendril>pulp. The distribution of PAs was in the order of fruit skin>leaf>stem≥tendril>pulp. 4. Seedlessness treatments increased the contents of polyphenols and antioxidant capacity in leaf, stem and tentril, while decreased them in skin and pulp.
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