论文标题:中小负荷运动对心理应激大鼠血清皮质酮、IL-2和血浆T淋巴细胞亚群的影响
The Effect of Low and Moderate Exercise on the Corticosterone, IL-2 and T-lymphocyte Subsets in Peripheral Blood of SD Rats after Being Received Psychological Stress
论文作者 尹剑春
论文导师 童昭岗,论文学位 硕士,论文专业 运动人体科学
论文单位 扬州大学,点击次数 75,论文页数 48页File Size1847k
2003-04-01论文网 http://www.lw23.com/lunwen_823790677/ 心理应激,运动,T淋巴细胞亚群,皮质酮,白细胞介素2
psychological stress,exercise,T lymphocyte subsets,corticosterone,IL-2
中小负荷运动对心理应激大鼠血清皮质酮、IL-2和血浆T淋巴细胞亚群的影响 心理神经免疫学是在本世纪70年代后期出现的新兴科学，它的使命是描述心身之间的关系，力图从分子水平上了解它们之间的联系，并运用这些知识预防，减轻人们的病患。随着该研究的深入发展，越来越多的学者开始重视对应激的研究，因为长期应激状态下可以通过神经内分泌系统，中枢神经系统影响机体免疫功能，危害人体的健康。采用何种干预手段对抗应激从而维持机体免疫功能的稳定成为现代心理神经免疫学研究的热点。 传统的干预手段大多采用认知和药物干预，这些方法固然有效，然而它们操作繁琐，价格昂贵，且操作权仅掌握在少数临床医生手中，如果有一种既简便又便宜且行之有效的干预方法的话，那将是心理神经免疫学的一大突破。 身体锻炼作为一种适合现代人的健身、健心的方法，恰恰为这一领域提供了新的科学的立足点，但是关于这一领域研究国内外研究甚少，尤其是身体锻炼对纯心理应激下的免疫功能的变化及其机制的研究更为少见。本论文试图采用Communication Box的经典心理应激模型，通过对运动大鼠血清皮质酮，IL-2和血浆T淋巴细胞亚群的检测从免疫学和神经内分泌学角度探讨这种有益身心作用的可能机制。 将54只SD雄性大鼠，随机分为安静对照组、心理应激组、应激+30min运动组，应激+60min运动组、30min运动组、60min运动组，电击组(除30min运动组和应激+30min运动组每组7只以外，其余每组8只) 结果发现：(1)与对照组相比，应激组大鼠血清IL-2水平显著低于对照组；CD3+CD4+CD8+水平虽没有显著差异但有下降的趋势，血清皮质酮含量显著高于对照组。这表明反复的心理应激引起大鼠较强的HPA轴反应对大鼠免疫功能有抑制作用。 (2)与对照组相比，30min和60min运动组血清IL-2水平和血浆T细胞亚群数目都显著高于对照组，而血清皮质酮含量显著低于对照组。中小负荷运动有效的提高了大鼠机体免疫功能并使HPA轴产生适应性变化。 (3)与应激组相比，应激+60Inin运动组的IL一2和CD3+，CD4+/CDS+水平显著高于应激组:应激+3 omin运动组CD4+/ CDS+水平没有显著差异，但并不低于应激组，皮质酮含t显著低于应激组.说明运动训练可以通过改变H队轴的应激激素的变化对抗心理应激造成的免疫功能的低下，且中等负荷对抗心理应激有着十分重要的意义。结论:反复的心理应激引起大鼠较强的心理反应并使大鼠免疫功能产生抑制，中小负荷运动对在心理应激下的大鼠免疫功能有较好的保护作用，且中等负荷运动更能提高大鼠抗应激能力，其可能机制在于中小负荷运动使得大鼠HPA轴产生较好的适应性，改变了应激激素在体内的分泌，从而维持了机体免疫功能的稳定.
Psycho-neuro-immunology( PNI )is a new science produced in 1970s, whose task is mainly describe the relationship between brain and body. The purpose is to understand then- relations from the level of molecule for relieving people" s disease. With the deep development of this research.more and more scholars begin to pay more attentions on the stress" research,for the reason that the body" s immune system can be influenced by the central nervous system and neurosecretory system during stress periods, which is usually "harmful to one" s health. What kind of intervene measures could be used to antagonize the stress and keep the stability of one"s immune system is becoming the top point in the PNI research.Traditional intervene measures often use congnition and medicine, however they can not be widely used for their high price and over-elaborated operation, also the right of operation often belongs to the few clinical doctors. If there has a convenient and effective intervene measures ,that could be a big breakthrough hi the PNI.As a suitable method for modern people , physical excise provide a new and scientific stand point in this field ,but why and what does it work? it is still not clear. In the experiment research .SDrats were divided into 7 groups ,one as control, one got the electrical footshock, and one received pure psychological stress only ,the other received forced swimming training for 6 weeks and psychological stress for 2 weeks in the later stage training, but the training last 30min and 60min separately. The IL-2 and corticosterone level and the number of T lymphocyte subsets in peripheral blood were measured. Result: Compared with the control group ,the IL-2 level of psychological stress was significantly lower than that of control; the number of CD3+ and CD4+/CD8+of psychological stress group had not significant change, but had the lower trend, while their corticosterone level was significantly higher than that of control.; the IL-2 and CD3+.CD4+/CD8+ levels of both 30min group and 60min group were significantly higher than that of control, while their corticosterone level was significantly lower than that of control; Compared with the stress group , the IL-2 and CD3+,CD4+/CD8+ levels of both 30min +stress group and 60min+stress group were significantly higher than that of stress group, while their corticosterone level was significantly lower than that of stress. Conclusion: The effect of exercise on psycho-neuro-immunology is likely related to the adaptation of HPA following chronic excise.
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