论文标题:电针对急性脑梗死大鼠神经可塑性影响的实验研究
The Experimental Study of the Effect of Electro Acupuncture to the Nervous Plasticity in Rats after Acute Cerebral Infarction
论文作者 刘波
论文导师 唐强,论文学位 硕士,论文专业 针灸推拿学
论文单位 黑龙江中医药大学,点击次数 70,论文页数 35页File Size4939k
2004-05-01论文网 http://www.lw23.com/lunwen_871220632/ 电针;脑梗死;神经元/突触
electro acupuncture;cerebral infarction;Neuron/synapse
目的：探讨电针对大鼠急性脑梗死后神经可塑性影响的物质基础及其发生机制。方法：采用线栓法闭塞大鼠大脑中动脉，制备局灶性脑缺血模型，用光镜、电镜、免疫组织化学方法观察电针“前三里”、“后三里”穴对大鼠梗死区域缺血损伤组织、坏死神经元和血管、突触、微管相关蛋白2(MAP-2)、突触生长素(SYN)等变化的影响。结果：电针可以改善神经功能评分和组织病理学指标，促进突触数目和结构的恢复，增加MAP-2和SYN的表达。结论：电针可对局灶性脑缺血Wistar大鼠产生明显的脑保护作用，促进脑缺血后突触功能的重建和发挥对脑组织神经细胞可塑性的调节作用。
Purpose: To investigate the effect of electro acupuncture to the structure basis of nervous plasticity and the recovery mechanism after acute cerebral infarction. Methods: A rat model of focal cerebral is chemia was made by filament occlusion of the middle cerebral artery. The effect of electro acupuncture in Qian san Li and Hou san Li to a rate"s change in infarction tissue, necrosis of neuron and vessel, synapse microtubule associated protein 2(MAP-2),and synaptophysin wore observed by electron scope, light scope ,and immunohistology staining . Results: Electro acupuncture could improve histopathologic indices, increase the number of synapse, and promote the recover of synapse"s structure, increase the expression of the SYN and map-2 .Conclusion: Electro acupuncture can produce a marked protecting effect on the brain in Wistar rats focal cerebral ischemia, regulate the plasticity of cerebral neuron.
|
