论文标题:桂花组织培养技术体系的研究
Study on the Technique System of Tissue Culture in Sweet Osmanthus (Osmanthus Fragrance Lour.)
论文作者 宋会访
论文导师 王彩云,论文学位 硕士,论文专业 园林植物与观赏园艺
论文单位 华中农业大学,点击次数 228,论文页数 44页File Size3102k
2004-06-01论文网 http://www.lw23.com/lunwen_95110117/ 桂花;组织培养;无菌苗
Sweet osmanthus; Tissue culture; Rapid propagation
桂花(Osmanthus fragrans Lour)为中国十大名花之一，又是优良的园林绿化树种和香花经济树种。本研究的主要目标是探索桂花组织培养快速繁殖的体系，以桂花的胚、新梢茎段、叶片等为外植体，着重探讨组培过程中最佳培养基配方及培养程序。主要研究结果如下： 1．桂花茎段和叶片以0.1％升汞为消毒试剂，最佳消毒时间分别为3min和2min。 2．桂花胚初代培养的最佳培养基为LMc+BA1.00mg／L+GA1.00mg／L，发芽率为100.00％，幼苗生长快。 3．利用桂花新梢茎段作为初代培养的外植体，最佳的取材时间为2月下旬，最佳培养基为LMc+KT 8.00mg／L+NAA0.10mg／L，萌芽率为70.7％。 4．继代增殖培养的最适培养基为LMc+TDZ0.50mg／L+NAA0.10 mg／L，其年增殖系数可达1.6~*10~6。 5．生根的最适培养基为1／2MS+NAA2.00mg／L，生根率可达95％以上，根系粗壮，生长迅速。 6．移栽以腐殖质土为培养基质，并以基本培养基1／2LMc为营养液进行叶面喷施，移栽成活率可达80.00％以上。 7．愈伤组织诱导的最佳培养基为MS+BA0.50mg／L+2，4-D0.10 mg／L，MS+BA0.50mg／L+NAA2.00 mg／L较好。暗培养比光培养更有利于愈伤的产生。
Sweet osmanthus (Osmanthus fragrans Lour) is one of ten traditional famous flowers in China and the excellent landscape trees that used for improving city environments and fragrant economic trees. The major object of this study is to exploit the technique systems of tissue culture and rapid propagation for sweet osmanthus. Using the embryo, fresh shoots and leaves as the explant, the optimal medium and culture programe are studied. The major results as following:1. The concentration of disinfectant mercuric chloride for stems and leaves of sweet osmanthus is 0.10%. The optimizing time of disinfection for stems is 3 minutes and leaves 2 minutes separately.2. The optimizing medium for embryo initial culture was LMc+ BA1.00mg/L+GA1.00 mg/L. The germination ratio was 100% with seedlings fast grow.3. Using the fresh shoots of sweet osmanthus as the explant for initial culture, the best season for selecting material is the last ten-day period of February. The optimizing medium for initial culture is LMc+KT8.00mg/L+NAA0.10mg/L. The germination ratio is 70.70%.4. The optimizing medium for subculture multiplication is LMc+TDZ0.5mg/L+ NAA0.10 mg/L. The average propagation coeffcient per year or yearly isl.6*106.5. The optimizing medium of rooting is l/2MS+NAA2.00mg/L, ratio of rooting is above 95.00%, with strong and quick-grow roots.6. Using humus soil as culture substrate and the base media of l/2LMc to foliar fertilization, the ratio of transplant viability is above 80.00%.7. The optimizing medium for callus induction is LMc+BA0.50mg/L+2, 4-D0.10 mg/L, medium of LMc+BA0.50mg/L+NAA2.00 mg/L is better. Comparing to the culture under light, the culture under dark can form callus more efficiently.
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