论文标题:金雀异黄素抑制增生性玻璃体视网膜病变的研究
Effects of Genistein on Traumatic Proliferative Vitreoretinopathy in Rabbits
论文作者
论文导师 姜德咏,论文学位 博士,论文专业 眼科学
论文单位 中南大学,点击次数 644,论文页数 70页File Size3132K
2006-05-01论文网 http://www.lw23.com/lunwen_98607/
Proliferative vitreoretinopathy; animal model; genistein; PDGF; bFGF; PCNA
[研究背景] 增生性玻璃体视网膜病变(proliferative vitreoretinopathy)是孔源性视网膜脱离和穿通性眼外伤及其术后眼组织的反应过程。在过去数十年间，尽管人们一直致力于更好地理解和处理PVR，它仍然是视网膜脱离手术失败的最主要原因。随着对PVR发病机制认识的不断深入，许多学者已开始尝试用药物来防治PVR，希望取得事半功倍的效果。 金雀异黄素(Genistein)是大豆异黄酮的主要成分。1987年，Akiyama等首次报道金雀异黄素是生长因子受体酪氨酸激酶的抑制剂。近年来，金雀异黄素因其抗肿瘤、抗新生血管等特性而受到广泛关注。多数体外试验证实，金雀异黄素能抑制视网膜色素上皮细胞(Retinal Pigment Epithelium)增殖、诱导其凋亡，并可抑制其分泌多种细胞因子。本实验旨在研究金雀异黄素对兔外伤性PVR的作用，及对PCNA、PDGF、bFGF表达的影响，为PVR的药物治疗提供理论依据。 [目的] 1．探讨金雀异黄素对外伤性PVR的抑制作用； 2．探讨金雀异黄素对外伤性PVR模型中PCNA、PDGF、bFGF表达的影响作用。 [方法] 1．用富含血小板血浆玻璃体腔注射制备兔PVR模型，分为五组：A组(阴性对照组)，B组(金雀异黄素2Hg组)，C组(金雀异黄素20pg组)，D组(金雀异黄素40pg组)，E组(阳性对照组)。在制模后第1d、3d、5d、7d、
[Background]Proliferative vitreoretinopathy (PVR) is a reactive process of the ocular tissue after perforating trauma, retinal detachment, and surgical manipulations. It is characterized by retinal pigment epithelium cells, neuroglial, and inflammative cells proliferate on the retina, under the retina and in the vitreous cavity. Proliferative vitreoretinopathy (PVR) is still the most common cause of failure of surgery for rhegmatogenous retinal detachment, despite the substantial effort that has been devoted to better understanding and managing this condition during the past 25 years, With the better understanding of the pathological mechanism of PVR, many ophthalmologists bigan to search for effective medicine treatment for PVR.Gnistein is the main component of soybean isoflavone. In 1987, Akiyama reported that genistein is the inhibitor of tyrosine kinase frist. In resent years, many people focused on genistein because of it"s effect on antitumor and antineovascularization. Many vitro experiments have proved that genistein can inhibit proliferation and induce apoptosis of retinal pigment epithelium cells, and provent retinal pigment epithelium cells from secreting many kinds of cytokines.Our study is to investigate the role of genistein in rabbit traumatic proliferative vitreoretinopathy and its influence on the expression of PDGF, bFGF, and PCNA, to provide theory proof for the medicine treatment of PVR.[Objective]1. To study the inhibition of genistein in traumatic proliferative vitreoratinopathy.2. To study the influence of genistein on expression of PDGF, bFGF, and PCNA in traumatic proliferative vitreoratinopathy model.[Method]1. Traumatic PVR models were induced in pigmented rabbits by intravitreal injection of platelet rich plasma. Models were divided in 5 groups: A group(negative control/DMSO 0.1 ml), B group(positive control/5-FU 1mg), C group(genistein 2μg), D group(genistein 20μg), E group(genistein 40μg). After surgery, the eyes were examined ophthalmoscopically on the 1st, 3rd,
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